FIGURE 3.

The highest enhancer activity by TGF-β and Wnt signalings in the C region of the TMEPAI gene. A, schematic presentation of deletion mutants for luciferase (Luc) reporters using the first intron of the TMEPAI gene. B–D, the reporter activity of each deletion mutant upon stimulation of TGF-β, expression of TCF7L2, or expression of β-catenin. HepG2 cells transfected with pGL3ti-A, pGL3ti-B, or pGL3ti-C were stimulated with TGF-β (B), cotransfected with TCF7L2 (C), or cotransfected with β-catenin (D). E, synergistic activation of pGL3ti-C by the combination of ALK5ca with lithium chloride. 293T cells transfected with pGL3ti-850 together with or without ALK5ca were cultured in the presence or absence of 20 mm lithium chloride.