FIGURE 5.
Importance of particular SBEs together with TTE in the C region of the TMEPAI gene. A, schematic presentation of point mutants for luciferase (Luc) assay using the C region. Each SBE is numbered from the 5′ upstream of the C region. B, requirement of three distal SBEs for full activation of the pGL3ti-C reporter. HepG2 cells transfected with each reporter and TCF7L2 were stimulated with TGF-β. C and D, loss of synergistic activation by introduction of mutations in three SBEs for pGL3ti-C (C) or pGL3ti-(SBE)3(TTE) (D). HepG2 cells transfected with plasmids described were stimulated with TGF-β.