TABLE 1.
Summary of PS1 LL1 and TM2 experimental mutant characterization
The extent of PS1 endoproteolysis, Aβ secretion, and processing of C99-6myc, NotchΔEMV-6myc, and N-cadherin CTF are indicated. ++, robust activity comparable with that of WT PS1. +, reduced activity relative to WT PS1. +/−, only weak activity. −, no activity.
| PS1 processing | Aβ generation | AICD generation | Notch processing | N-Cadherin processing | |
|---|---|---|---|---|---|
| PS1-wt | ++ | ++ | ++ | ++ | ++ |
| LL1Ins | ++ | ++ | ++ | ++ | ++ |
| MutA | ++ | + | + | − | − |
| MutB | + | − | − | − | − |
| 8aaLL1 | − | − | − | − | − |
| 16aaLL1 | + | − | − | − | − |
| LL1-10/18Ala | + | + | + | − | − |
| LL1-13/18Ala | ++ | + | + | − | − |
| LL1-19/27Ala | + | + | + | − | − |
| LL1-28/32Ala | ++ | ++ | ++ | ++ | ++ |
| TM2-1/3Ala | ++ | + | + | − | − |
| TM2-aa1/4 | +/− | − | − | − | − |
| TM2-aa6/15 | ++ | ++ | ++ | ++ | − |
| TM2-aa16/22 | − | − | − | + | − |
| TM2-18/22Ala | + | + | + | + | − |
| TM2-aa16/22Res | ++ | ++ | ++ | ++ | ++ |