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Transcription of the gal regulon promoters by stationary phase (σ38) RNA polymerase. In vitro transcription assays were performed on a supercoiled pRPGSM DNA (6) template in the presence of the heat unstable nucleoid protein HU (80 nM) and cAMP. The presence or absence of CRP is indicated at the bottom of each lane. Experiments in lanes 1 and 2 were performed with σ70 RNAP holoenzyme, and those in lanes 3 and 4 with reconstituted σ38 RNAP holoenzyme.
