FIGURE 2.

Purification of the recombinant Tay1 protein. Tay1p-6HN was purified by TALON Superflow metal affinity chromatography (heparin input), loaded onto a heparin column, and eluted by a linear 0–1.5 m NaCl gradient, and the proteins were analyzed by 10% SDS-PAGE. Shown are peak fractions eluted from the heparin column. Fractions 10–16 were pooled, dialyzed, and used for subsequent experiments.