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Reduced coenzyme A-coupled assay determination of Erf2 mutant protein Activity
NA, not applicable.
| Erf2 Protein | Vmax | Km | kcat | kcat/Km |
|---|---|---|---|---|
| pmol/min/μg | μm | min−1 | min−1 μm−1 | |
| WT | 46.0 ± 10.0 | 53.0 ± 25 | 0.74 ± 0.10 | 0.014 |
| R185A | 83 ± 25.0 | 57.0 ± 38 | 1.14 ± 0.14 | 0.02 |
| H201A | 9.7 ± 1.0 | NA | ||
| C203S | 11.1 ± 0.5 | NA | ||
| C209S | 84 ± 5.0 | 14.5 ± 3 | 1.31 ± 0.18 | 0.09 |
| F218S | 25 ± 1.0 | 11.0 ± 2 | 0.33 ± 0.04 | 0.03 |
| Boiled | 3.9 ± 2.9 | NA |
