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. 2010 Oct 2;285(49):38125–38140. doi: 10.1074/jbc.M110.148650

FIGURE 5.

FIGURE 5.

Myocardial activity of PP2A and PP1 after RV pressure overloading. Myocardial homogenates for these assays were prepared from the same animals as those used to prepare the immunoblots in Fig. 4. A, PP2A activity was measured using an immunoprecipitation assay kit (catalog number 17-313; Upstate Biotech) as described under “Experimental Procedures.” B, PP1 activity was determined using the PSP assay system (catalog number P0780S; New England Biolabs), as is also described under “Experimental Procedures.” *, p < 0.05 by one-way ANOVA with Bonferroni post hoc analysis.