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. 2010 Oct 2;285(49):38125–38140. doi: 10.1074/jbc.M110.148650

FIGURE 9.

FIGURE 9.

Intrinsic microtubule stability. These immunoblots were prepared, using our peptide antibodies to Tyr-tubulin, Glu-tubulin, and Δ2-tubulin (38), from myocardial homogenates from the same groups of mice used for Fig. 8. The increases relative to control in the post-translationally modified Glu and Δ2 α-tubulin isoforms reflect greater microtubule stability in vivo, because these modifications occur only in the microtubule-assembled αβ-tubulin heterodimers (38, 39). For this and two other sets of immunoblots, for the left set of blots, the densitometric ratio of MAP4/control signals was 1.11 ± 0.06 for Tyr-tubulin, 1.66 ± 0.05 for Glu-tubulin, and 2.64 ± 0.12 for Δ2-tubulin; for PP2A/control, it was 1.13 ± 0.08 for Tyr-tubulin, 1.77 ± 0.06 for Glu-tubulin, and 2.55 ± 0.13 for Δ2-tubulin. For the right set of blots the densitometric ratio of MAP4/control signals was 1.06 ± 0.03 for Tyr-tubulin, 1.70 ± 0.31 for Glu-tubulin, and 2.70 ± 0.51 for Δ2-tubulin; for PP1/control, it was 1.16 ± 0.14 for Tyr-tubulin, 1.58 ± 0.03 for Glu-tubulin, and 2.55 ± 0.12 for Δ2-tubulin.