FIGURE 4.

Native acrylamide gels of PDE5A1(C149S) fragments. A, native gel of PDE5A1(C149S) (amino acids 89–518). Samples were treated with various concentrations of cGMP or with 2 mm cAMP and 25 mm DTT. B, native gels of PDE5A1 fragments of various lengths. Samples were treated with 25 mm DTT in the presence (+) or absence (−) of 2 mm cGMP. Lanes 1 and 2, PDE5A1-(89–518); lanes 3 and 4, PDE5A1-(98–518); lanes 5 and 6, PDE5A1-(116–518); lanes 7 and 8, PDE5A1-(126–518); lanes 9 and 10, PDE5A1-(142–518).