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. 2010 Sep 24;285(49):38157–38166. doi: 10.1074/jbc.M110.167486

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FIGURE 4. — In vivo effect of SLIMP knockdown by RNA interference. A, constitutive and ubiquitous knockdown of SLIMP causes a decrease in adult viability at 25 °C and at 29 °C (***, p < 0.001). B, wing-restricted SLIMP depletion. RNAi_SLIMP strains crossed at 29 °C with the nubbin-GAL4; UAS-dcr2 driver show severe tissue damage. Scale bars correspond to 500 μm. C, SLIMP silencing in the L3-L4 wing area. RNAi_SLIMP strains are crossed with the patched-GAL4 driver that restricts the RNAi expression in the region flanked by longitudinal veins L3 and L4. Some individuals present a partial or total loss of the anterior cross vein (marked with an arrowhead). Graphs show the averages of all the measurements in percentage with S.E. when compared with the paternal line (**, p < 0.01; ***, p < 0.001). Scale bars correspond to 500 μm.