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Identification of CPA6 peptide substrates by quantitative peptidomics. Peptides were extracted from mouse brain, digested with different amounts of purified CPA6, labeled with isotopic tags (D0 = 1 nm CPA6; D3 = 10 nm CPA6; D9 = 100 nm CPA6; D12 = no enzyme) and analyzed by LC-MS/MS for quantitative peptidomics analysis. Examples of representative data are shown for (A) non-substrates, (B) good substrates, (C) weak substrates, and (D) products.
