TABLE 2.
Good substrates of CPA6
| Protein name | Peptide name | Sequence | Za | T | Obs M | Theor M | ppm | Ratio, CPA6/no enzyme |
||
|---|---|---|---|---|---|---|---|---|---|---|
| Low CPA6 | Med CPA6 | High CPA6 | ||||||||
| Chromogranin B | 600–613 | QYDGVAELDQLLHY | 2 | 1 | 1662.80 | 1662.79 | 4 | 1.03 | 0.69 | 0.00 |
| Chromogranin B | 64–86 | SGKEVKGEEKGENQNSKFEVRLL | 6 | 5 | 2604.38 | 2604.35 | 11 | 0.76 | 0.33 | 0.00 |
| Proenkephalin | Leu-Enkephalin | YGGFL | 1 | 1 | 555.27 | 555.27 | −7 | 1.10 | 0.75 | 0.11 |
| Proenkephalin | Met-Enkephalin | YGGFM | 1 | 1 | 573.23 | 573.23 | 0 | 0.81 | 0.52 | 0.06 |
| Proenkephalin | Heptapeptide | YGGFMRF | 2 | 1 | 876.37 | 876.39 | −25 | 0.73 | 0.00 | 0.00 |
| Proenkephalin | Octapeptide | YGGFMRSL | 2 | 1 | 929.43 | 929.45 | −23 | 0.95 | 0.48 | 0.00 |
| Pro-SAAS | Little SAAS | SLSAASAPLVETSTPLRL | 2 | 1 | 1811.99 | 1812.01 | −12 | 0.95 | 0.41 | 0.08 |
| Secretogranin II | 300–316 | ESKDQLSEDASKVITYL | 3 | 3 | 1924.94 | 1924.96 | −11 | 0.79 | 0.42 | 0.00 |
a Z, charge; T, number of TMAB tags; Obs M, observed mass; Theor M, theoretical mass; ppm, difference in parts per million between observed mass and theoretical mass; ratio indicates the peak intensity observed for peptide incubated with CPA6 divided by the peak intensity for the same peptide incubated without enzyme.