FIGURE 1.

TAFI inhibition by protein inhibitors. TAFI (0.37 μm) cleaved the substrate Hippuryl-Arg at a constant rate, but its activity was strongly impaired upon incubation with increasing amounts of protein inhibitors, PCI (···), LCI (·−·) or TCI (−) (panel A). Complex formation between TAFI (2.5 μg) and the protein inhibitors (1 μg) was further studied by non-denaturing PAGE (panel B). The enzyme (2.5 μg) alone and each of the inhibitors (5 μg) were included as controls in the gel. PCI alone failed to enter the gel and TCI stained poorly.