Skip to main content
. 2010 Oct 5;285(49):38260–38269. doi: 10.1074/jbc.M110.138735

FIGURE 1.

FIGURE 1.

Rsf-1 expression induces growth arrest in non-transformed OSE cells. IOSE-80pc cells were used for studying the biological effects of Rsf-1 overexpression. A, Western blot analysis shows Rsf-1-V5 protein band in treated cells 48 h after virus transduction by using anti-Rsf-1 and anti-V5 antibodies. B, immunocytochemistry demonstrates nuclear Rsf-1-V5 immunoreactivity in almost all treated cells 48 h after virus transduction. Cells treated with empty vector virus were used as controls. C, mRNA expression levels of Rsf-1 at different time points were measured by real-time quantitative PCR. D, cell growth analysis demonstrated a growth arrest in Rsf-1/V5 virus-transduced IOSE-80pc cells as compared with control virus-transduced cells. E, the percentage of annexin V-stained apoptotic cells increased in Rsf-1 virus-transduced cells as compared with control virus-transduced cells at days 3 and 4 after virus transduction (p < 0.001). Error bar: one standard error.