FIGURE 5.

Interaction of the full-length Rsf-1 and SNF2H is required for Rsf-1 to induce growth arrest in nontransformed RK3E cells. A, most SNF2H knockdown cells exhibited undetectable staining of pCHK2 and γH2AX. B, Western blot analysis shows an efficient reduction of SNF2H protein by a SNF2H-specific siRNA pool. C, co-events were those cells showing down-regulation in both SNF2H and pCHK2 or both SNF2H and γH2AX. D, growth curve analyses in Rsf-1 inducible RK3E cells transfected with anti-SNF2H siRNA or buffer alone (mock control). E, schematic presentation of the full-length Rsf-1 and its different deletion mutants (D1, D3, D4, and D6). F, Western blot analysis showed expression of deletion mutants in RK3E cells 48 h after induction. G, growth curve analyses demonstrate no significant effects of deletion mutants on cellular proliferation as compared with the full-length Rsf-1. Error bar: one standard error.