FIGURE 8.

TP53 mutation confers a selective growth advantage for Rsf-1-overexpressing RK3E cells. A, Rsf-1-expressing RK3E cells, which carry mutant (R175H) and wild type p53, were mixed at a ratio of 1:25 and grown in low serum (0.5%) medium for 30 days. The diffuse Rsf-1 expression was demonstrated by immunostaining for V5. Cells with p53 mutant in the mixed culture were stained with an anti-Xpress (XP) antibody that only recognizes mutant p53 tagged with an XP epitope. A mixed culture under Rsf-1 gene off was used as the control. B, the population of p53 mutant cells in the mixed culture was measured at indicated time points by counting XP-positive cells. Error bar: one standard error.