FIGURE 8.

Effect of mutations in the LDEVFL motif on co-purification of DrrA and DrrB. A, Western blot analysis using anti-DrrA and anti-DrrB antibodies. Lanes 1–3, Sup, DrrAB proteins solubilized from membrane fraction with 1% DDM; lanes 4–6, 500 mm fraction 1 (fr.1), the first 500 mm imidazole elution fraction; lanes 7–9, 500 mm fraction 2 (fr.2), the second 500 mm imidazole elution fraction. The intensity of bands in Western blots was determined by densitometric scanning, and co-purification efficiencies were plotted in histograms shown in B and C. This experiment was repeated three times. Error bars in B and C, S.D.