TABLE 2.

Effect of mutations in the LDEVFL or CREEM motif on doxorubicin efflux

Doxorubicin efflux was measured as described under “Experimental Procedures.” Based on the data shown in supplemental Fig. S4, the efficiency of efflux in each LDEVFL or CREEM mutant was calculated, as described under “Results.” The values shown are the average of the data obtained from three independent experiments. The top shows the percentage efflux in various mutations in the LDEVFL motif. The bottom shows the percentage efflux in mutations in the CREEM.

Wild type	Vector	L303V	D304N	V306A/F307A/L308A	L310A/T311A/G312A	Δ LDEVFL
%	%	%	%	%	%	%
100	30.6	31.5	78	26.9	43.5	35.8
Wild type	Vector	3E-3D	3E-3G	4E-4Q	5E-5G	Δ CREEM
100	36.5	100	97	109	54.5	55