FIGURE 4.

Identification of scFv epitopes. a, immobilized biotinylated peptides, including an alanine scan of the last 20 amino acids of IRF-1 LDSLLTPVRLPSIQAIPCAP, were incubated with 1 μg/ml scFv1–5. scFv binding was detected with anti-His. A peptide containing the epitope for scFvN is labeled as Co, and the data are representative of two independent experiments. b, as in a except using scFvN. c, diagrammatic representation of the amino acid motifs targeted by scFv1–5.