FIGURE 4.

Constitutive activation depends on the binding of N-glycan in the Q595N/R597T mutant. Three amino acid residues starting from Gln-595 in the αIIb chain were mutated to various sequences. Fbg binding to the cells in the presence of 1 mm Ca²⁺/1 mm Mg²⁺ with a control antibody (solid column) or with PT25-2 (hatched column) and in the presence of 1 mm Mn²⁺ with a control antibody (gray column) was examined. MFI, mean fluorescent intensity. Error bars indicate S.E.