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. 2010 Oct 11;285(49):38438–38443. doi: 10.1074/jbc.M110.183913

FIGURE 4.

FIGURE 4.

Effects of different combinations of osmolytes on the refolding rate of ArPKD domains. A, measuring the refolding rate for ArPKD domains using a two-pulse stretching/relaxation protocol. Three examples obtained with time delays of 0.1, 3, and 10 s are shown. B, plot of the folding probability of as a function of the time delay between stretching pulses obtained in PBS (black squares), 1 m urea (red circles), 1 m urea + 1 m sorbitol (purple triangles), and 1 m urea + 0.5 m TMAO (green diamonds). The lines correspond to fits of the data to the function Pf(t) = 1 − exp (−βot), using the following folding rate constants: βo, 0.58 ± 0.16 s −1 in PBS, 0.07 ± 0.04 s−1 in 1 m urea, 0.52 ± 0.19 s−1 in 1 m urea + 1 m sorbitol, and 0.24 ± 0.07 s−1 in 1 m urea + 0.5 m TMAO.