TABLE 1.
Peak elution conductivities of thrombin and its complexes from an analytical heparin Sepharose column and dissociation constants for fluorescein-γ′
ND, not determined.
| Proteins | Peak conductivity | Dissociation constant for fluorescein-γ′ |
|---|---|---|
| mS/cm | μm | |
| Plasma thrombin | 54.4 | 0.175 ± 0.025 |
| Plasma thrombin-α1ATpitts complex | 33.0 | ND |
| Cleaved plasma thrombin-α1ATpitts complex | 24.8 | ND |
| Plasma thrombin-PPACK | 55.9 | ND |
| Recombinant thrombin | 60.1 | 0.201 ± 0.015 |
| Recombinant R93E thrombin | 39.4 | 14.1 ± 0.6 |
| Recombinant thrombin- α1ATpitts complex | 39.7 | ND |
| Recombinant R93E thrombin- α1ATpitts complex | 35.7 | ND |