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. 2010 Oct 6;285(49):38666–38673. doi: 10.1074/jbc.M110.155036

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — STIM1-dependent and STIM1-independent function of receptor-activated TRPC channels. Black traces are for HEK cells transfected with GFP and M3 receptors only and show the native receptor-stimulated Ca²⁺ influx. HEK cells were also transfected with M3 receptors, the indicated TRPC channel mutants, and empty vector (green traces) with wild-type STIM1 (blue traces) or with STIM1(K684E,K685E) (S1(KK/EE); red traces). The cells in Ca²⁺-free medium were stimulated with 0.1 mm carbachol (Carb) and then incubated with medium containing carbachol and 2 mm Ca²⁺ to assay Ca²⁺ influx. A, TRPC3(D697K,D698K) (TRPC3(DD/KK), C3(DD/KK)); B, TRPC4(E648K,E649K) (TRPC4(EE/KK), C4(EE/KK)); C, TRPC5(D651K,E652K) (TRPC5(DE/KK), C5(DE/KK)); D, TRPC6(E755K,E756K) (TRPC6(EE/KK), C6(EE/KK)). The traces are the mean ± S.E. of at least five cells and are representative of three more experiments with similar results.