FIGURE 8.

NVP binding kinetics: apparent binding rates of nevirapine (k_app-NVP). RT (30 nm active sites) was first incubated in RT buffer with 30 nm Td₃₁/Cy3-Pd₁₈. This solution was rapidly mixed with an equal volume of saturating NVP (40 μm) for the indicated times before then being mixed with saturating dATP (50 μm) and MgCl₂ (10 mm). The reactions were quenched with 250 mm EDTA, pH 8.0. Reaction products were fit to a single exponential decay equation to obtain observed binding rates. The k_app for RT WT (■) was 2.3 ± 0.6 s⁻¹, the k_app for p66_N348I/p51_N348I (□) was 1.3 ± 0.4 s⁻¹, the k_app for p66_N348I/p51_WT (●) was 2.0 ± 0.6 s⁻¹, and the k_app for p66_WT/p51_N348I (○) was 0.6 ± 0.1 s⁻¹.