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. 2010 Oct 4;285(49):38710–38719. doi: 10.1074/jbc.M110.137414

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — CAPS1 is associated with the Golgi membrane via the PH domain. A–C, accumulated expression of CAPS1(PH)-YPet-HA (the PH domain of CAPS1 C-terminally fused to the fluorescent protein YPet and the HA epitope tag) around Stx6-immunopositive Golgi structures of PC12 cells. A, CAPS1(PH)-YPet-HA (green); B, Stx6 (red); C, merged image. Scale bars, 20 μm. D–I, subcellular distribution of six deletion derivatives of CAPS1-YPet-HA expressed in PC12 cells; immunostaining for HA. D, wild-type CAPS1-YPet-HA; E, PH domain alone (PH)-YPet-HA; F, PH domain and Munc13-1-homologous domain (ΔN-Ter)-YPet-HA; G, the region from the first amino acid to the PH domain (ΔC-Ter)-YPet-HA; H, C2 and PH domain (ΔN/C-Ter)-YPet-HA; I, C2 domain-skipped CAPS1 (ΔC2)-YPet-HA. Scale bars, 20 μm.