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. 2010 Oct 4;285(49):38710–38719. doi: 10.1074/jbc.M110.137414

FIGURE 2.

FIGURE 2.

Endogenous CAPS1 is associated with the Golgi membrane. A and B, electron micrograms of submicrosomal fractions affinity-purified using immune magnetic beads. Cerebellar microsomal fractions of P21 mice were incubated with magnetic beads coated with anti-CAPS1 antibody (A) or anti-VAMP2 antibody (B), and immunoaffinity-purified submicrosomal fractions were subjected to electron microscopic observation. Scale bars, 200 nm. C, Western blot analysis of submicrosomal fractions affinity-purified using normal rabbit IgG, anti-CAPS1 antibody, or anti-VAMP2 antibody immunomagnetic beads. Each immunopurified fraction was immunoblotted with anti-CAPS1, anti-VAMP2, anti-VAMP4, anti-SNAP25, anti-Bcl-2, anti-BiP/GRP78, anti-pan-ARF (1D9), anti-GM130, anti-GS28, anti-p115, anti-Stx5, anti-Stx6, anti-Stx16, and anti-Vti1a antibodies. Anti-pan-ARF (1D9) antibody recognizes all of the ARF family proteins (supplemental Fig. S3).