Skip to main content
. 2010 Dec;24(12):5043–5051. doi: 10.1096/fj.10-168724

Figure 2.

Figure 2.

A) GC synthesis in thymocytes is regulated by androgens. Expression of genes encoding steroidogenic enzymes involved in GC synthesis was analyzed by qPCR in thymocytes from castrated mice (n=8) as compared to sham-operated controls (n=8). B) LUC activity stimulated by thymocytes derived from castrated mice vs. sham-operated control mice, as determined by the GC reporter gene coculture assay. C) Expression of genes encoding GC steroidogenic enzymes from castrated + testosterone treated mice (n=8), as compared to vehicle-treated castrated mice (n=8). D) CS secretion by cultured (20 h) thymocytes from castrated+testosterone-treated mice vs. castrated + vehicle-alone-treated mice, as determined by ELISA. E) LUC activity stimulated by thymocytes derived from castrated + testosterone-treated mice vs. castrated+vehicle-alone-treated mice. Control, reporter cells (transfected HEK293 cells) without thymocytes; T, reporter cells plus thymocytes. *P < 0.05; **P < 0.001. NS, not significant.