Figure 2.
Cavβs form both homo- and heterooligomers. A) Venus-labeled β3 (lanes C1, 1) β1b (lanes C2, 2), β2d (lanes C3, 3) (0.8 μg each), or mVenus (control, lane C4; 0.4 μg) were expressed in the absence (lanes C1–C3) or presence (lanes 1–3, C4) of Flag-β3 (0.5 μg) in Cos7 cells, immunoprecipitated by an anti-Flag antibody, and immunoblotted with anti-Flag and anti-GFP antibody. B, C) Venus-labeled Cavβs were also coimmunoprecipitated with Flag-β2d (B) or Flag-β1b (C). (Note that the presence of low-molecular-mass bands recognized by an anti-GFP antibody may represent degraded Cavβs).