Figure 3.

Mφ ablation in Cd11b-DTR mice prevents normal kidney repair following IRI. A) Percentage of kidney cells that are CD11b⁺, NK1.1⁻, Ly6G⁻ Mφs, as assessed by flow cytometry. B) CD11b⁺ and F4/80⁺ cells in the kidney following IRI, quantified by immunostaining of kidney sections. C) Photomicrographs of F4/80 staining and PAS staining of kidneys at d 6 post-IRI following no Mφ ablation (vehicle) or Mφ ablation with DT from d 3 to 6. D) F4/80 area in control kidneys or d 6 post-IRI kidneys following ablation. E) Effect of Mφ ablation from d 3 to 6 post-IRI on plasma creatinine. Note that following Mφ ablation there is a higher creatinine level on d 6 than in mice with normal Mφ compliment. F) tubule injury score in d 6 post-IRI kidneys following ablation. Scale bars = 100 μm. *P < 0.05.