Skip to main content
. 2010 Dec;24(12):4648–4659. doi: 10.1096/fj.10-162230

Figure 2.

Figure 2.

ANGPTL4 is regulated by HIF-1α in osteoclasts. A) Hypoxic induction of ANGPTL4 mRNA (open bars, n=7) and protein (shaded bars, n=4). B) Induction of ANGPTL4 mRNA by 24 h exposure to CoCl2 (100 μM), desferrioxamine (DFO, 100 μM), and l-mimosine (100 μM). C) Effect of siRNA targeting HIF-1α (H1), HIF-2α (H2), or HIF-1α scrambled control (scr) on expression of HIF-1α (open bars) or HIF-2α (shaded bars) mRNA; relative expression under hypoxia, normalized to ACTB. D) Western blot showing hypoxic expression of HIF-1α and HIF-2α (5× overexposed compared with HIF-1α) in response to siRNA targeting HIF-1α, HIF-2α, or HIF-1α scrambled control. E) Effect of HIF siRNA on ANGPTL4 mRNA expression in normoxic and hypoxic osteoclasts. *P < 0.05; **P < 0.01; ***P < 0.001.