Figure 7.

Osteoblasts and ANGPTL4. A, B) Effect of 24 h exposure to 2% O₂ (shaded bars) on ANGPTL4 expression assessed by real-time PCR (A) and ELISA (B) vs. the normoxic (open bars) control. Mean fold hypoxic induction is indicated over the bars for each cell type (n/a, not calculated, normoxic levels undetectable). *P < 0.05; **P < 0.01; ***P < 0.001. C) Western blot showing hypoxic induction of ANGPTL4 in the same cell panel and detecting full-length ANGPTL4 of the predicted 41- to 45-kDa molecular mass [FL(41–45)], full-length ANGPTL4 of 50–55 kDa at which it normally runs in reducing conditions [FL(55)], and the FLD domain at 20–35 kDa (FLD). OC, in vitro differentiated osteoclasts; MON, CD14⁺ monocytes; THP1, monocytic cell line; HOb, primary human osteoblasts; GCTB stromal cells, mononuclear stromal component of GCTB; MG-63 and Saos2, osteoblastic cell lines.