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. 2010 Nov 16;11:85. doi: 10.1186/1471-2199-11-85

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Copyright ©2010 Lin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Assays of the transcriptional activities of the pentapeptide and its tandem repeats. (A) Transcriptional assays. The pentapeptide, WYDWW, and its tandem repeats were expressed as fusions to the specific DNA-binding protein, LexA, and the abilities of the resulting fusion proteins to turn on the reporter genes (LEU2 and lacZ), which are controlled by distinct LexA-responsive promoters, were tested. (B) Quantitative assays of β-gal activity. (C) Western blot analysis of the expressions of LexA fusion proteins. Upper panel, LexA fusion protein; lower panel, PGK (as a loading control). The numbers (circled) in A, B, and C represent the constructs shown in A.