Figure 5.

Assays of the transcriptional activity of W₇ using the Gal4-based system. (A) Transcriptional assays. W₇ and (WYDWW)₂ were expressed as fusions to the Gal4-DBD, and the abilities of the resulting fusion proteins to turn on the reporter genes (HIS3 and MEL1), which are controlled by distinct Gal4-responsive promoters, were tested. (B) Quantitative assays of β-gal activity. (C) Western blot analysis of the expressions of Gal4-DBD fusion proteins. Upper panel, LexA fusion protein; lower panel, PGK (as a loading control). The numbers (circled) in A, B, and C denote the constructs shown in A.