Figure 7.

Identification of the interacting partners of W₇ using a two-hybrid system. (A) Transcriptional assay. TBP, TFIIB, and TFIIH were expressed as fusions to LexA, and the abilities of the resulting fusions to turn on the reporter genes (LEU2 and lacZ) were tested. (B) Western blot analysis of the expressions of LexA fusion proteins. Upper panel, LexA fusion protein; lower panel, PGK (as a loading control). The numbers (circled) in A and B denote the constructs shown in A. (C) Two-hybrid assays. TBP, TFIIB, and TFIIH were expressed as fusions to LexA, and the abilities of the resulting fusions to specifically interact with W₇ and turn on the reporter genes (LEU2 and lacZ) were tested. For clarity, the bait and prey fusion proteins encoded by the constructs are depicted in parentheses following the vector names.