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Primer sequences used in PCR for amplifying porB1A and porB1B genes
| Primers | Sequences (5' to 3')* | Products and sizes |
|---|---|---|
| porB1A/1B | F: ATGAAAAAATCCCTGATTGCC | 981 bp for entire porB1A gene, and 1044 bp for entire porB1B gene |
| R: TTAGAATTTGTGGCGCAGAAC | ||
| porB1A/1B-D | F1: GCCATTTGGCAGTTGGAACA | 520 bp for partial sequence of porB1A gene, and 201 bp and 583 bp for partial sequence of porB1B gene |
| F2: GATACGGCGAAGGCACTAAA | ||
| R: CTTCGGTTTGAGAGTTGTGC |
* F: forward primer, R: reverse primer
