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. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: Biomaterials. 2010 Nov 10;32(4):1193–1203. doi: 10.1016/j.biomaterials.2010.08.079

Fig. 9 — Intracellular localization of pDNA delivered by RPC-bPEI_0.8kDa, bPEI_25kDa, and bPEI_0.8kDa in polyplex-transfected HEK293 cells 4 hr after transfection: (a) accumulated images and (b) center-sectional images. Nuclei, pDNA, and acidic vesicles were distinguished using Hoechst 33342 (blue), YOYO-1 (green), and LysoTracker® dye (red).

Fig. 9 — Intracellular localization of pDNA delivered by RPC-bPEI_0.8kDa, bPEI_25kDa, and bPEI_0.8kDa in polyplex-transfected HEK293 cells 4 hr after transfection: (a) accumulated images and (b) center-sectional images. Nuclei, pDNA, and acidic vesicles were distinguished using Hoechst 33342 (blue), YOYO-1 (green), and LysoTracker® dye (red).