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. 2011 Jan 27;21(2):140–149. doi: 10.1111/j.1750-3639.2010.00430.x

Figure 2.

Figure 2

Methylene blue (MB) does not significantly increase mitochondrial function. (A) Representative Western blots of protein obtained from a mitochondrial enriched fraction from brains of MB‐treated (n = 6) and control 3xTg‐AD (n = 5) mice and probed with COX‐IV and VDAC antibodies as a loading control. (B) Densitometric analysis of the blots revealed no significant change in the COX‐IV levels. (C) No changes in cytochrome c oxidase activity were observed in MB‐treated 3xTg‐AD mice (n = 17) compared with 3xTg‐AD mice (n = 12) on the control diet. (D) ATP levels were measured in the cytosolic fraction and no significant changes were observed in MB‐treated 3xTg‐AD mice (n = 17) compared with 3xTg‐AD mice on the control diet (n = 13). (E) Kinetic readings were taken to measure ATP production in the mitochondrial enriched fraction energized with glutamate and malate by luciferase assay. ATP production rates were not significantly changed in MB‐treated 3xTg‐AD mice (n = 15) compared with 3xTg‐AD mice on the control diet (n = 11). Data were analyzed using t‐test analysis.