Figure 4.

Methylene blue (MB) does not affect tau pathology in 3xTg‐AD. (A) Representative Western blots of proteins extracted from brains of 3xTg‐AD mice and probed with human tau specific antibody HT7, phospho‐specific antibody AT270 (which recognize tau phosphorylated at Thr181) with β‐actin as a loading control. (B) Densitometric analysis of blots (normalized to β‐actin) indicates that MB has no effect on tau accumulation or phosphorylation. Data obtained from five treated and five untreated 3xTg‐AD mice. (C–D) Representative microphotographs of CA1 pyramidal neurons stained with anti‐tau antibody HT7 indicate that MB does not alter tau mislocalization. Five mice/treatment were analyzed. Data are presented as ± standard error of the mean and analyzed by t‐test analysis.