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. 2010 Oct 29;10(5):565–579. doi: 10.1159/000317254

Fig. 6.

Fig. 6

Role of p53 in induction of apoptosis in MEF p53 (–/–) and Capan-1 and Capan-2 cells. a Restoration of TMZ sensitivity in p53 (–/–) MEF transiently transfected with CMV-p53. TMZ-induced apoptosis in p53 (–/–) transfectants was determined by TUNEL assay. Transfectants were treated with 30 μM BG or 500 μM TMZ plus 30 μM BG and subjected to TUNEL analysis. Bars depict the average from 2 independent experiments ± standard error of approximately 1,000 cells scored in each experiment. b Restoration of TMZ sensitivity in Capan-1 cells transiently transfected with CMV-p53. TMZ-induced apoptosis in the transfectants was determined by TUNEL assay. Transfectants were treated with 30 μM BG or 500 μM TMZ plus 30 μM BG and subjected to TUNEL analysis. Bars depict the average from 2 independent experiments ± standard error of approximately 1,000 cells scored in each experiment. c Reversal of TMZ sensitivity in Capan-2 cells transiently transfected with p53 siRNA. TMZ-induced apoptosis in Capan-2 transfectants was determined by TUNEL assay. Transfectants (control siRNA or p53 siRNA) were treated with 30 μM BG alone or 500 μM TMZ plus 30 μM BG and subjected to TUNEL analysis. Approximately 1,000 cells in total were scored in each experiment (p < 0.05). d Western blot analysis of the transient transfectants of Capan-2 (vector siRNA or p53 siRNA) left untreated or treated with BG alone, 500 μM TMZ alone, and 500 μM TMZ plus 30 μM BG for p53, BAX, and p21 proteins. β-Actin was used as the loading control. UT = Untreated.