Figure 2.

Relationship between CD4+ T cell number and proliferation level (by Ki67 expression) after depletion of CD4+ T cells with antibody: (a) three uninfected RMs and (b) three uninfected SMs were treated with repeated infusion of the humanized anti-CD4 monoclonal antibody Cdr-OKT4A-huIgG1clone 12F11, and the fractions of CD3+,CD4+ T cells monitored over time by flow cytometry (as previously described; Klatt et al. 2008). Both Ab-depleted species show a significant negative correlation between CD4+ T cell count and the proportion of cells Ki67+ (Spearman correlation), even in the absence of virus. The relationship between total CD4+ T cell number and level of proliferation [r(T), solid line] is likewise fitted with an exponential function and the parameters, as well as the p-values, are shown in table 2.