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. 2010 Aug 29;59(12):3090–3098. doi: 10.2337/db10-0624

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — RIP-Cre transgenic lines display Cre-mediated recombination in multiple regions of the brain. Adult brains were sliced into four or five coronal sections and subjected to whole mount X-gal staining. Images of individual brain slices from each sectioning plane are available in supplementary Figs. 1–4. A: Sagittal and coronal views of mouse brain (adapted from Allen Mouse Brain Atlas, http://www.brain-map.org/) (29). A dashed vertical line marks coronal sectioning plane spanning the hypothalamic region of the brain. B–E: Images of coronal brain slices located on the left side of the sectioning plane in the sagittal view in A. B: R26R^wt/lacZ littermate control mice (n = 17) lacked X-gal staining in the brain. The cortex (CTX) and hypothalamus (HY) are labeled and correspond to regions marked on the coronal view in A. C: RIP-Cre^Mgn;R26R^wt/lacZ mice (n = 8) showed X-gal staining throughout the brain with high signal intensity in the mid-brain and ventral regions. D: RIP-Cre^Herr;R26R^wt/lacZ mice (n = 14) showed weaker, punctate X-gal staining throughout the brain without obvious regionalization. E: RIP-Cre/ERT;R26R^wt/lacZ mice (n = 4) injected intraperitoneally with three 2-mg doses of tamoxifen over a 5-day period displayed strong, punctate X-gal staining throughout the brain with expression pattern more restricted than in RIP-Cre^Mgn; R26R^wt/lacZ mice. Brains from littermate controls injected with corn oil vehicle were negative for X-gal staining (data not shown). F–I: Whole-mount X-gal staining of pancreas from R26R^wt/lacZ in F, RIP-Cre^Mgn; R26R^wt/lacZ in H, RIP-Cre^Herr;R26R^wt/lacZ in G, and RIP-Cre/ERT;R26R^wt/lacZ mice in I. (A high-quality digital representation of this figure is available in the online issue.)