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. 2010 Sep 14;59(12):3108–3116. doi: 10.2337/db09-1886

FIG. 5.

FIG. 5.

Gene transfer of GLP-1 and HGF/NK1 increase β-cell proliferation in db/db mice. A: Pancreatic sections from db/db mice infected with dsAAV–eGFP, dsAAV–GLP, or dsAAV–NK1 were stained for insulin (red) and the proliferation marker, Ki67 (green). Arrowheads indicate cells that are double-positive for insulin and Ki67. B: Quantification of Ki67-positive β-cells, expressed as the mean percentage of Ki67-positive, insulin-positive cells in 20–40 individual islets from each group. *P < 0.05. C: Pancreatic sections from db/db mice infected with dsAAV–eGFP, dsAAV–GLP, or dsAAV–NK1 were stained for insulin (red) and subjected to TUNEL staining (green). Arrowheads indicate cells that are double-positive for insulin and TUNEL. D: Quantification of TUNEL-positive β-cells, expressed as the mean percentage of TUNEL-positive, insulin-positive cells in 20–40 individual islets from each treatment group. *P < 0.002. For all images, magnification = ×40, scale bars = 100 μm. (A high-quality digital representation of this figure is available in the online issue.)