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a) Amino acid sequence of A. flos-aquae GvpA and corresponding PSIPRED12 secondary structure prediction (where H indicates α–helix, E β–sheet, and C coil) with associated estimates of confidence (where 0 = low, 9 = high).
b) BLAST13 results for residue conservation among GvpA in cyanobacteria (middle row) and cyanobacteria, other bacteria, and archaea (bottom row). Letters indicate absolute conservation, + indicates conservative substitutions, and blank spaces no conservation.
