Physical characteristics of NMJ and muscle fibers of Hb9:CHT mice. (A and B) Sample NMJ of CHT+/+ (A) and CHT+/+;Hb9:CHT (B) from the Flexor digitorum brevis (FDB). (C) The NMJ area is not different between genotypes (CHT+/+ n=4, Hb9:CHT n=5; two-tailed t-test, P>0.1). (D and E) Sample images of cross-sections of gastrocnemius muscles stained for SDH. (F) Cross-sectional area of each fiber type is not different between genotypes (CHT+/+ n=3, Hb9:CHT n=5; two-way ANOVA, P>0.1). (G) Cross-sectional area of the entire gastrocnemius is also not different between genotypes (CHT+/+ n=4, Hb9:CHT n=5; two-tailed t-test, P>0.1).