Figure 2.

Cytotoxicity assays of (a) sgc8c-Dox conjugates, (b) free Dox, (c) free sgc8c, and (d) TDO5-Dox conjugates with CCRF-CEM cell line. The cells (2 × 104 cells/well) were incubated with Dox or aptamer-Dox conjugates (0 to 2.5 μM) in culture medium without FBS at 37 °C, 5% CO2 for 2 h. After drug treatment, cells were subsequently grown in fresh medium (10% FBS) for 48 h. The cytotoxicity was then measured by MTT assay.