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. Author manuscript; available in PMC: 2010 Nov 28.
Published in final edited form as: Chembiochem. 2009 Mar 23;10(5):862–868. doi: 10.1002/cbic.200800805

Figure 3.

Figure 3

Confocal images display the distribution of sgc8c-Dox conjugates inside CCRF-CEM cells at different time points: A) 30 min, B) 1 h, and C) 2 h. The cells (106) were incubated with sgc8c-Dox (0.5 μM) and transferrin-Alexa633 (60 nM) in culture medium without FBS at 37 °C, 5% CO2 for 2 h. From left to right, the fluorescence images were monitored for sgc8c-Dox, transferrin-alexa633, overlay of these two channels, and bright field channel, respectively. Transferrin-Alexa633 will both bind to the surface and internalize to the endosomal compartment of CCRF-CEM cells.