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. Author manuscript; available in PMC: 2010 Nov 28.
Published in final edited form as: Chembiochem. 2009 Mar 23;10(5):862–868. doi: 10.1002/cbic.200800805

Figure 4.

Figure 4

Quantitative analysis of A) Dox and B) sgc8c-Dox inside CCRF-CEM cells. The cells (105 cells) were incubated with Dox or aptamer-Dox conjugates in culture medium without FBS at 37 °C, 5% CO2 for 2 h. A) Dashed and solid lines represent the fluorescence signal from Dox before and after trypsin treatment, respectively. Trypsin was applied to remove the membrane-bound Dox. B) Fluorescence signal from sgc8c-Dox inside cells determined by flow cytometry. All the signals have been subtracted by the fluorescence intensity from TDO5-Dox nonspecifically uptaken by CCRF-CEM cells.