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Flow cytometry assay for the binding of biotin-labeled TDO5 and sgc8c with three different cell lines: CCRF-CEM, NB-4, and Ramos cells. Cells (105) were incubated with biotin-labeled TDO5 and sgc8c at 37 °C for 20 min in 100 μL culture medium without FBS. After washing twice, cells were mixed with streptavidin-(R-phycoerythrin) (20 min on ice), and the fluorescence was determined by flow cytometry.
