Figure 1.

Treatment of 2DG led to ER stress in primary rabbit chondrocytes. (A) Chondrocytes were untreated or treated with 5 mM 2DG for the indicated time periods in rabbit articular chondrocytes. (B) Chondrocytes were untreated or treated with specific concentrations of 2DG for 24 h in rabbit articular chondrocytes. (A, B) Expressions of glucose-regulated protein of 94 kDa (grp94) were detected by a Western blot analysis. Expressions of β-actin were used as a loading control. The data represent a typical experiment, and similar results were obtained from four independent experiments.