Skip to main content
. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: Cell Metab. 2010 Oct 6;12(4):398–410. doi: 10.1016/j.cmet.2010.08.013

Figure 6. NHR-25 ligand-binding domain binds phosphoinositides.

Figure 6

Mammalian SF-1, an nhr-25 homolog, has been shown to bind phosphoinositide species. To test the ability of NHR-25 to bind these lipids, we generated and purified recombinant NHR-25 ligand binding domain. Incubation of this protein with PI(4,5)P and PI(3,4,5)P alters retention of the protein in ion exchange chromatography (A) indicating the protein has bound the lipid. Incubation of each phohsphoinositide species with NHR-25 ligand binding domain protein induced ~50% of the protein to bind lipid (B).